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Inventi Impact: Biomedical Analysis

Articles

  • Inventi:pba/112/14
    ANALYSIS OF THERAPEUTIC EFFECT OF ILEX HAINANENSIS MERR. EXTRACT ON NONALCOHOLIC FATTY LIVER DISEASE THROUGH URINE METABOLITE PROFILING BY ULTRAPERFORMANCE LIQUID CHROMATOGRAPHY/QUADRUPOLE TIME OF FLIGHT MASS SPECTROMETRY
    30-Jun-2014 Research 2014 : July - September
    Jing-jing Li, Jie Yang, Wei-xi Cui, Xiao-qing Chen, Gang-ling Chen, Xiao-dong Wen, Qiang Wang

    Nonalcoholic fatty liver disease (NAFLD), the most common form of chronic liver disease, is increased worldwide in parallel with\nthe obesity epidemic. Our previous studies have showed that the extract of I. hainanensis (EIH) can prevent NAFLD in rat fed\nwith high-fat diet. In this work, we aimed to find biomarkers of NAFLD and investigate the therapeutic effects of EIH. NAFLD\nmodel was induced in male Sprague-Dawley rats by high-fat diet. The NAFLD rats were administered EIH orally (250mg/kg)\nfor two weeks. After the experimental period, samples of 24 h urine were collected and analyzed by ultraperformance liquid\nchromatography/quadrupole time of flight mass spectrometry (UPLC-Q-TOF). Orthogonal partial least squares analysis (OPLSs)\nmodels were built to find biomarkers ofNAFLD and investigate the therapeutic effects of EIH. 22 metabolites, which are distributed\nin several metabolic pathways, were identified as potential biomarkers of NAFLD. Taking these biomarkers as screening indexes,\nEIH could reverse the pathological process of NAFLD through regulating the disturbed pathway of metabolism. The metabolomic\nresults not only supply a systematic view of the development and progression of NAFLD but also provide a theoretical basis for the\nprevention or treatment of NAFLD.

    How to Cite this Article
    CC Compliant Citation: Jing-jing Li, Jie Yang, Wei-xi Cui, et al., â??Analysis of Therapeutic Effect of Ilex hainanensis Merr. Extract on\nNonalcoholic Fatty Liver Disease through Urine Metabolite Profiling by Ultraperformance Liquid Chromatography/Quadrupole Time of\nFlight Mass Spectrometry,â? Evidence-Based Complementary and Alternative Medicine, vol. 2013, Article ID 451975, 12 pages, 2013.\ndoi:10.1155/2013/451975.\nCopyright © 2013 Jing-jing Li et al. This is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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